Example Capillary Measurement SOP

  1. Plug the CapiScope HVCS USB plug into the laptop.
  2. image0Click on the CapiScope HVCS desktop icon.
  3. For the CapiScope Select Profile box click OK
  4. image1Click the image2 record button (or f12 key). Click again to stop.
  5. image3Open the Image sequence dialog box.
  6. Enter a new base filename and path for recordings. (Use the image4 browse button at right to browse and create new folders).
  7. Click OK
  8. Press the image5 start button to start the camera.
  9. Focus using focus ring (sublingual) or top positioner (nailfold) and adjust brightness.
  10. image6Add oil if viewing nailfold.
  11. Repeat 9 and locate measuring site.
  12. Press image7 record button to start recording loop.
  13. Watch time elapsed as shown in bottom line of the status box.
  14. Press the pause button to stop recording.
  15. Goto 8 to start next recording with the same base filename.

Analysing Video

  1. Open the video file.
  2. Press the lower image8 play button, or drag the horizontal scrollbar to the first good image in focus.
  3. Select menu edit, duplicate image in new window.
  4. Press image9 measurement button to show the dimension line buttons.
  5. image10Press the image11 dimension line button.
  6. Draw vessel diameter measuring lines across apex of each capillary in final row.
  7. Press TAB key until first dimension line is highlighted.
  8. Press the image12 find width button.
  9. image13Press accept ( or return key )if the greylevel window shows a clean “V” and the sides of the V have been detected ok.
  10. Repeat 7-9 for the remaining capillaries.
  11. Save the Image with the dimension lines.
  12. Save the Diameter Calculation Results.
  13. Go back to the video file.
  14. Use Tools, Automatic Movement Correction.
  15. Press <ctrl> l to play the recording in a continuous loop.
  16. Draw a dimension line on the first capillary in the final row. Draw the line in the direction of flow.
  17. Press the image14 stop button.
  18. Use Window, Linescan to open the linescan window.
  19. Activate the video file window and press image15 play button.
  20. image16This will produce a velocity trace in the linescan window as a black line.
  21. Select a portion or all the trace for averaging by dragging while holding down the left mouse button.
  22. Use the TAB key to step through the different traces in the linescan window until the status box top line shows 0:cbv. (capilllary blood cell velocity)
  23. Press = button or = key to get the average cbv.
  24. Activate the video file window and press TAB to activate the dimension line. Press delete to delete it.
  25. Repeat steps 15-17, 19-24 for each other capillary to measure in the final row.
  26. Save the chart results window with the cbv averages.